[split] building a transition matrix for ion channels

[satyam2007]

hey. Can someone help me in building a transition matrix for ion channels? I was coding for the markov chain in potassium ion channels but I am not able to figure out how to code for transition matrix.

[scidam]

Transition matrix is just a matrix of probabilities of events like that: "if system is in state i, what is the probability it will be in the state j at next (time) step. (P[i,j]) ".Some probabilities could be assigned to zero from some expert knowledge. Other values should be estimated based on observed data. It is important to know what are the system states in this case, and how many observations you have.
It should be easy, I think, if you have dataset (observations) covering all possible transitions from i to j.